A. ENO1, ENO1-mut and MBP-1-constructs with translation starts (ATG) and mutated (ATG→ATC) sites in ENO1-mut at positions 374 and 383 indicated. The mutations introduce Bcl I and Cla I restriction enzyme sites respectively. B. Size determination of cDNA-constructs in pIRES-EGFP-vector by NotI/EcoRI-digest. C. Confirmation of mutations in ENO1-mut DNA by ClaI and BclI digests.