Figure 6

Staining for p65/RELA, phospho-Akt and cIAP-2 on serial biopsy sections. Serial sections from a moderate dysplasia (A–C) or adenocarcinoma (D–F) underwent immunohistochemical staining with antibodies directed against p65/RELA (A and D), phospho-Akt (B and E) or cIAP-2 (C and F). Cells with nuclear localization of p65/RELA (arrows in A) correlated with regions of increased staining for phospho-Akt and cIAP-2 in this moderate dysplasia but the correlation was not consistently observed. High levels of cytoplasmic p65/RELA staining in the adenocarcinoma corresponded with faint to moderate staining for phospho-Akt and cIAP-2 (D–F). There was a statistically significant correlation between cIAP-2 and phospho-Akt staining intensity at all pathology grades.