EGF mediated activation in ME180AP-1 CellSensor. EGF effectively stimulated the EGFR/AP-1 pathway in ME180 cervical cancer cells. Cells expressing β-lactamase were detected using a fluorescent plate reader (Panel A) or under fluorescent microscopy (Panel B). β-lactamase was measured using Invitrogen's LiveBLAzer™ FRET B/G assay. Ratios represent the mean of (± SEM) eight independent data points (n = 8).