Table 1 Primer sequences used to amplify EphB4 and the control housekeeping genes PBGD and HPRT 1 with expected size of the corresponding PCR product in base pairs (bp) and the annealing temperature used in the reaction.
From: Investigation of the expression of the EphB4 receptor tyrosine kinase in prostate carcinoma
Primer | Sequence 5'-3' | Product | Annealing | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
EphB4 F | CCC | CAG | GGA | AGA | AGG | AGA | GCT | G | Â | Â | Â |
EphB4 R | GCC | CAC | GAG | CTG | GAT | GAC | TGT | G |  | 250 bp | 68°C |
PBGD F | CTT | TCC | AAG | CGG | AGC | CAT | GTC | TGG | Â | Â | Â |
PBGD R | CAT | GAG | GGT | TTT | CCC | GCT | TGC | AGA |  | 377 bp | 68°C |
HPRT F | GGC | TAT | AAA | TTC | TTT | GCT | GAC | CTG | CTG | Â | Â |
HPRT R | CAA | AGT | CTG | CAT | TGT | TTT | GCC | AGT | GTC | 230 bp | 67°C |