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Table 1 Primer sequences used to amplify EphB4 and the control housekeeping genes PBGD and HPRT 1 with expected size of the corresponding PCR product in base pairs (bp) and the annealing temperature used in the reaction.

From: Investigation of the expression of the EphB4 receptor tyrosine kinase in prostate carcinoma

Primer Sequence 5'-3' Product Annealing
EphB4 F CCC CAG GGA AGA AGG AGA GCT G    
EphB4 R GCC CAC GAG CTG GAT GAC TGT G   250 bp 68°C
PBGD F CTT TCC AAG CGG AGC CAT GTC TGG    
PBGD R CAT GAG GGT TTT CCC GCT TGC AGA   377 bp 68°C
HPRT F GGC TAT AAA TTC TTT GCT GAC CTG CTG   
HPRT R CAA AGT CTG CAT TGT TTT GCC AGT GTC 230 bp 67°C
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