Lack of GIRK1 and 2 expression in hamster PNEC cells. Top panel: Pulmonary neuroendocrine cells were isolated from fetal hamsters on the 15th day of gestation. Neither GIRK1 nor GIRK2 was expressed in normal PNEC. Bottom panel: Tissue was isolated from adult hamster brain to determine if the lack of expression in PNEC was not due to species differences in PCR primers. Both GIRK1 and GIRK2 were expressed in hamster brain. Cyclophilin, used as a positive reaction control was seen in the samples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK1-441 bp; GIRK2-438 bp; cyclophilin-216 bp, M-100 bp.