Figure 1
Activation of PBMC by PstS1 is dose-dependent. PBMCs were stimulated for 7 days with different PstS1 concentrations in a range from 0.1 μg/ml to 100 μg/ml. A 4-hour chromium release assay against T-24 bladder tumor cells. Effector-target cell ratio of 40:1 B IFN-γ release was determined by ELISA from supernatants of the stimulated PBMCs C PBMC proliferation was measured by overnight 3H-Thymidine incorporation assay (1 μCi/2 × 105 cells). One representative experiment out of 3–6 is shown (mean ± SD). n.d. = not detectable