Figure 1
From: Absence of annexin I expression in B-cell non-Hodgkin's lymphomas and cell lines
Expression of annexin I in human B-cell lymphoma cell lines. Whole cell extracts were prepared from HeLa (lane 1), REH (lane 2), HPB-Null (lane 3), DW-10 (lane 4), Nalm-6 (lane 5), Namalwa (lane 6), PBE-1 (lane 7), Ramos (lane 8), Raji (lane 9), OMA-BL-1 (lane 10), WI-L2 (lane 11), TK-6 (lane 12) and adenoid (lane 13) in panel A and extracts prepared from normal B-cells obtained from PBL (lane 1), DHL-16 (lane 2) and Raji (lane 3) cells in panel B were used to measure expression of annexin I. Extracts equivalent to 20 μg of protein were subjected to SDS-gel electrophoresis and immunoblotting as described in Materials and Methods. Protein blots were incubated with a 1:2000 dilution of polyclonal anti-annexin I (α646) antiserum and 1:500 dilution of anti-human PGK antiserum (α35). Positions of the molecular weight markers, PGK and annexin I are indicated.