CD155 is recruited to the leading edge of migrating cells and colocalizes with actin and αv-integrin. (a) HT1080 cells were grown to near confluency on a matrigel substrate. A linear wound was made with a 20 g needle and cells allowed to recover for 3 h. Cells were fixed and immunostained for CD155. The majority of CD155 appeared to localize to the leading edge of migrating cells. (b) CD155 colocalized extensively with actin and αv-integrin at the leading edge of HT1080 cells migrating on a matrigel substrate. CD155 did not appear to colocalize with the growth-factor receptor ErbB2.