Transformation and scattering activities of the receptor tyrosine kinase RON/Stk in rodent fibroblasts and lack of regulation by the jaagsiekte sheep retrovirus receptor, Hyal2

Table 1 Assay for functional expression of Hyal2 proteins in 208F/Stk cells transduced with Hyal2-expressing retroviral vectors

Source of vector used to transduce cells	LAPSN(PJ4) vector titer (AP⁺ FFU/ml) on cells:
	208F/Stk c12	208F/Stk c13
No vector	<10	<10
PT67/LhHyal1SN c16	<10	<10
PT67/LhHyal2SN c16	2,000	2,000
PT67/LrHyal2SN c2	800	600
PT67/LrHyal2SN c12	600	600
PT67/LmHyal2SN c7	<10	<10
PT67/LmHyal2SN c8	<10	<10

208F/Stk c12 and 208F/Stk c13 cells were exposed to vectors from the indicated vector-producing packaging cell lines and were exposed to G418 to select for expression of the vector. The cells were then exposed to JSRV-pseudotype LAPSN vector from PJ4/LAPSN cells and the apparent titers were determined.

ISSN: 1471-2407