Figure 3

Comparison of B-Raf V599E, B-Raf and C-Raf specific antibody responses between control patients or melanoma patients. The comparison has been performed between 119 control sera with 371 sera of melanoma patients tested for B-Raf V599E and 272 sera of melanoma patients tested for B-Raf and C-Raf. Titers correspond to the last serial dilution of the patients sera exceeding a cut-off OD₄₀₅ of 0.275. Compared to the background values in the absence of antigen (control -; melanoma -), the difference is not significant for control patients (P = 0.232; 0.471; 0.4054 for B-Raf V599E, B-Raf wt and C-Raf respectively, two tailed Mann-Whitney U test) and significant for melanoma patients (P = 0.012; 0.0009; 0.0022 for B-Raf V599E, B-Raf wt and C-Raf respectively, two tailed Mann-Whitney U test).