Recombinant S100A4 induces NF-κB transactivation. A, II-11b cells were transiently transfected with an NF-κB reporter construct and incubated with or without 4 μg/ml rS100A4 and 50 μM zFA-fmk for 48 hours. Luciferase activity was expressed as fold induction of activity compared to the corresponding untreated control. Data are mean values ± S.D. The results represent at least three independent experiments performed in duplicate. *, p < 0.001 and **, p < 0.01 as compared to untreated control. ***, p < 0.005 as compared to rS100A4-treatment without zFA-fmk. B, Western blot analysis of NF-κB p65 and phospho-IκBα (Ser32/36) in total cell lysates from II-11b cells, untreated or treated with 100 u/ml IFN-γ and/or 20 μg/ml rS100A4 for 24 and 48 hours as indicated.