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Table 2 Astrocytoma associated genes validated by real-time PCR.

From: Identification of astrocytoma associated genes including cell surface markers

Tag sequence Symbol Gene/Ontology Mean tumora Mean normalb % SAGEc % RT-PCRd
   Low grade astrocytomas (grade II)     
TGGGATTCCC CHI3L2 Chitinase 3-like 2 / cartilage biogenesis, extra-cellular 10.8 0 25 57
ATCTGCTCGG NMB Neuromedin B / signal transduction, neuropeptide 9.1 0.1 38 57
GTCAGAACTT   cDNA FLJ22528 / unknown 5.8 0.3 38 43
GTACGGAGAT VCAM1 Vascular cell adhesion molecule 1/ cell adhesion 4.4 0.2 50 29
   Anaplastic astrocytomas (grade III)     
TGGGATTCCC CHI3L2 Chitinase 3-like 2 / cartilage biogenesis, extra-cellular 12.3 0 30 63
GTGCGGAGGA SAA1 Serum amyloid A1/ inflammatory response, lipid metabolism, extra-cellular 11.4 0 20 50
TTTGCACTTT SMOC1 Secreted modular calcium-binding 1/ calcium ion binding 6.0 0 50 50
CCATTGTGGA ADORA3 Adenosine A3 receptor / signal transduction, G-protein coupled receptor 4.1 0.1 60 50
ATCTGCTCGG NMB Neuromedin B/ signal transduction, neuropeptide 9.1 0.1 40 81
CGCGGCGGCG CRF C1q-related factor / function unknown 7.8 0.1 70 56
CCCAGTAAGA CSRP2 Cysteine and glycine-rich protein 2 / cell differentiation/growth, LIM domain protein 9.4 0.2 80 44
GTACGGAGAT VCAM1 Vascular cell adhesion molecule 1/ cell adhesion 3.0 0.2 20 69
   Glioblastomas (grade IV)     
ACTGTCGCCA AQP1 aquaporin 1 / water transport, ion channel 5.8 0 22 53
ATGTAGAGTG TYMS thymidylate synthetase / pyrimidine metabolism 7.1 0 67 71
CTCAGCAATG TOP2A topoisomerase (DNA) II alpha (170 kD) / DNA metabolism, isomerase 4.2 0 44 88
GTCAACAGTA ABCC3 ATP-binding cassette, subfamily C, member 3/ multidrug resistance, transport protein 12.3 0 89 94
GTGCGGAGGA SAA1 serum amyloid A1/ inflammatory response, lipid metabolism, extra-cellular 14.3 0 44 53
TGGGATTCCC CHI3L2 chitinase 3-like 2 / cartilage biogenesis, extra-cellular 31.3 0 67 100
ATCTGCTCGG NMB Neuromedin B / signal transduction, neuropeptide 4.6 1 44 53
GTTTATGGAT MGP Matrix Gla protein /cartilage condensation, ossification, extra-cellular 7 2 56 35
  1. All counts are normalized per 100,000 tags. aAverage tag count per individual tumor SAGE library. bAverage tag count per individual normal neural tissue SAGE library. cPercentage of tumor SAGE libraries with an expression of at least 5 or more counts. dPercentage of tumor samples in an independent panel of tumors with at least a 5-fold of more over-expression when compared to neural normal tissue samples in real-time PCR analysis.