Figure 1
From: BAK overexpression mediates p53-independent apoptosis inducing effects on human gastric cancer cells
BAK gene overexpression mediated by gene transfer. A, Restrictive enzyme reaction assay after recombinant pcDNA-BAK digested with Xho I+Hind III (Lane 2); Hind III (Lane 3); Xho I (Lane 4); The blank pcDNA-BAK (Lane 5) and DNA marker λ DNA/Hind III + EcoR I (Lane 1) were used as controls. B, Reverse transcription polymerase chain reaction assay of BAK mRNA levels in untransferred (Lane 1), pcDNA3 transferred (Lane 2) and pcDNA-BAK transferred (Lane 3) gastric cancer cells. The expression level of α-Tubulin was used as a control. C, Western Blotting analysis of BAK protein levels in untransferred (Lane 3), pcDNA3 transferred (Lane 2) and pcDNA-BAK transferred (Lane 1) gastric cancer cells. The expression level of α-Tubulin was used as a control.