Table 2 PCR conditions for sequencing analysis of the C22orf2 gene
From: Is the gene encoding Chibby implicated as a tumour suppressor in colorectal cancer ?
Exon | Primers (5'-3') | Annealing temperature (°C) | PCR product (bp) |
|---|---|---|---|
2 | ex2F: GGCATAAGGTCAGTGATCCAG | 58 | 236 |
ex2R: TTCAGAGACACGCCTCAGCAC | |||
3–4 | ex3F: GGTCATATTGTTGGCGGAAG | 58 | 490 |
ex4R: CCAAACTGGCATTGAGGAGA | |||
5 | ex5F: CCAGCAGCATCAGAGAAGTG | 56 | 245 |
ex5R: AAAAGCACTCAGCCACATCC |