Expression of progesterone metabolizing enzyme genes (AKR1C1, AKR1C2, AKR1C3, SRD5A1, SRD5A2) is altered in human breast carcinoma

Michael J Lewis; John P Wiebe; J Godfrey Heathcote

doi:10.1186/1471-2407-4-27

Table 2 Expression levels of progesterone metabolizing enzymes (standardized against 18S mRNA) in tumorous and non-tumorous (Normal) resected breast tissues from 11 patients (described in Table 1).

From: Expression of progesterone metabolizing enzyme genes (AKR1C1, AKR1C2, AKR1C3, SRD5A1, SRD5A2) is altered in human breast carcinoma

Patient No.	5αR1 (SRD5A1) ^a		5αR2 (SRD5A2) ^b		20α-HSO (AKR1C1) ^a		3α-HSO-3 (AKR1C2) ^c		3α-HSO-2 (AKR1C3) ^a
	Normal	Tumor	Normal	Tumor	Normal	Tumor	Normal	Tumor	Normal	Tumor
1	1.68 ± 0.06	5.51 ± 0.30	0.99 ± 0.02	1.82 ± 0.10	0.76 ± 0.05	0.79 ± 0.11	0.16 ± 0.02	0.1 ± 0.01^d	1.25 ± 0.05	1.11 ± 0.09
2	0.20 ± 0.01	3.42 ± 0.44	0.11 ± 0.02	3.49 ± 0.03	3.77 ± 0.26	0.31 ± 0.03	3.24 ± 0.09	0.18 ± 0.02	3.81 ± 0.13	0.49 ± 0.02
3	0.64 ± 0.06	1.26 ± 0.08	0.48 ± 0.05	1.79 ± 0.09	4.31 ± 0.28	1.12 ± 0.05	3.78 ± 0.33	0.89 ± 0.05	4.01 ± 0.03	1.12 ± 0.20
4	0.88 ± 0.12	2.68 ± 0.12	0.28 ± 0.05	0.20 ± 0.02	7.41 ± 0.25	0.42 ± 0.02	7.20 ± 1.09	0.12 ± 0.01	7.77 ± 0.93	0.65 ± 0.07
5	0.38 ± 0.02	1.44 ± 0.10	0.92 ± 0.16	4.06 ± 0.33	6.07 ± 0.40	0.19 ± 0.03	6.94 ± 0.41	0.17 ± 0.02	6.54 ± 0.28	2.99 ± 0.17
6	0.66 ± 0.02	5.08 ± 0.12	0.94 ± 0.05	1.91 ± 0.15	6.04 ± 1.07	2.42 ± 0.07	5.75 ± 0.98	2.18 ± 0.10	8.31 ± 0.48	1.97 ± 0.09
7	0.20 ± 0.01	4.40 ± 0.46	0.26 ± 0.02	2.28 ± 0.12	4.79 ± 0.60	1.78 ± 0.06	6.01 ± 1.09	2.39 ± 0.03	6.07 ± 0.27	2.52 ± 0.15
8	0.58 ± 0.08	4.70 ± 0.12	0.65 ± 0.10	0.77 ± 0.02	1.51 ± 0.05	1.71 ± 0.13	1.89 ± 0.06	5.26 ± 0.88	2.83 ± 0.35	6.05 ± 0.42
9	0.20 ± 0.01	2.58 ± 0.12	0.38 ± 0.11	2.41 ± 0.24	4.70 ± 0.83	1.50 ± 0.16	5.59 ± 0.25	0.67 ± 0.14	6.63 ± 0.54	1.47 ± 0.10
10	1.08 ± 0.28	4.32 ± 0.22	0.61 ± 0.09	2.34 ± 0.14	5.50 ± 0.51	0.68 ± 0.09	4.66 ± 0.42	0.72 ± 0.14	5.25 ± 0.11	1.52 ± 0.14
11	1.32 ± 0.18	3.08 ± 0.26	1.22 ± 0.15	2.46 ± 0.09	6.04 ± 0.95	0.56 ± 0.07	6.32 ± 1.01	0.42 ± 0.07	5.78 ± 0.10	1.04 ± 0.08
Mean (±SEM)	0.709 (0.147)	3.496 (0.43)	0.614 (0.105)	2.139 (0.326)	4.627 (0.604)	1.044 (0.217)	4.685 (0.667)	1.191 (0.472)	5.296 (0.644)	1.905 (0.473)

Values were obtained from the average of duplicate PCRs run on the same gel and assays were repeated three times for each gene, using a fresh cDNA sample each time. Values are presented as mean ± SEM, n = 3. ^a Values were obtained by dividing the calculated intensity of 5αR1 or HSO bands (27 cycles) by the calculated intensity of the 18S band (12 cycles). ^b Values were obtained by dividing the calculated intensity of 5αR2 bands (33 cycles) by the calculated intensity of the 18S band (12 cycles). ^c Values obtained from very faint bands, calculated as <0.1, were adjusted to 0.1.

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