|
Patient No.
|
5αR1 (SRD5A1) a
|
5αR2 (SRD5A2) b
|
20α-HSO (AKR1C1) a
|
3α-HSO-3 (AKR1C2) c
|
3α-HSO-2 (AKR1C3) a
|
|---|
| |
Normal
|
Tumor
|
Normal
|
Tumor
|
Normal
|
Tumor
|
Normal
|
Tumor
|
Normal
|
Tumor
|
|---|
|
1
|
1.68 ± 0.06
|
5.51 ± 0.30
|
0.99 ± 0.02
|
1.82 ± 0.10
|
0.76 ± 0.05
|
0.79 ± 0.11
|
0.16 ± 0.02
|
0.1 ± 0.01d
|
1.25 ± 0.05
|
1.11 ± 0.09
|
|
2
|
0.20 ± 0.01
|
3.42 ± 0.44
|
0.11 ± 0.02
|
3.49 ± 0.03
|
3.77 ± 0.26
|
0.31 ± 0.03
|
3.24 ± 0.09
|
0.18 ± 0.02
|
3.81 ± 0.13
|
0.49 ± 0.02
|
|
3
|
0.64 ± 0.06
|
1.26 ± 0.08
|
0.48 ± 0.05
|
1.79 ± 0.09
|
4.31 ± 0.28
|
1.12 ± 0.05
|
3.78 ± 0.33
|
0.89 ± 0.05
|
4.01 ± 0.03
|
1.12 ± 0.20
|
|
4
|
0.88 ± 0.12
|
2.68 ± 0.12
|
0.28 ± 0.05
|
0.20 ± 0.02
|
7.41 ± 0.25
|
0.42 ± 0.02
|
7.20 ± 1.09
|
0.12 ± 0.01
|
7.77 ± 0.93
|
0.65 ± 0.07
|
|
5
|
0.38 ± 0.02
|
1.44 ± 0.10
|
0.92 ± 0.16
|
4.06 ± 0.33
|
6.07 ± 0.40
|
0.19 ± 0.03
|
6.94 ± 0.41
|
0.17 ± 0.02
|
6.54 ± 0.28
|
2.99 ± 0.17
|
|
6
|
0.66 ± 0.02
|
5.08 ± 0.12
|
0.94 ± 0.05
|
1.91 ± 0.15
|
6.04 ± 1.07
|
2.42 ± 0.07
|
5.75 ± 0.98
|
2.18 ± 0.10
|
8.31 ± 0.48
|
1.97 ± 0.09
|
|
7
|
0.20 ± 0.01
|
4.40 ± 0.46
|
0.26 ± 0.02
|
2.28 ± 0.12
|
4.79 ± 0.60
|
1.78 ± 0.06
|
6.01 ± 1.09
|
2.39 ± 0.03
|
6.07 ± 0.27
|
2.52 ± 0.15
|
|
8
|
0.58 ± 0.08
|
4.70 ± 0.12
|
0.65 ± 0.10
|
0.77 ± 0.02
|
1.51 ± 0.05
|
1.71 ± 0.13
|
1.89 ± 0.06
|
5.26 ± 0.88
|
2.83 ± 0.35
|
6.05 ± 0.42
|
|
9
|
0.20 ± 0.01
|
2.58 ± 0.12
|
0.38 ± 0.11
|
2.41 ± 0.24
|
4.70 ± 0.83
|
1.50 ± 0.16
|
5.59 ± 0.25
|
0.67 ± 0.14
|
6.63 ± 0.54
|
1.47 ± 0.10
|
|
10
|
1.08 ± 0.28
|
4.32 ± 0.22
|
0.61 ± 0.09
|
2.34 ± 0.14
|
5.50 ± 0.51
|
0.68 ± 0.09
|
4.66 ± 0.42
|
0.72 ± 0.14
|
5.25 ± 0.11
|
1.52 ± 0.14
|
|
11
|
1.32 ± 0.18
|
3.08 ± 0.26
|
1.22 ± 0.15
|
2.46 ± 0.09
|
6.04 ± 0.95
|
0.56 ± 0.07
|
6.32 ± 1.01
|
0.42 ± 0.07
|
5.78 ± 0.10
|
1.04 ± 0.08
|
|
Mean (±SEM)
|
0.709 (0.147)
|
3.496 (0.43)
|
0.614 (0.105)
|
2.139 (0.326)
|
4.627 (0.604)
|
1.044 (0.217)
|
4.685 (0.667)
|
1.191 (0.472)
|
5.296 (0.644)
|
1.905 (0.473)
|
- Values were obtained from the average of duplicate PCRs run on the same gel and assays were repeated three times for each gene, using a fresh cDNA sample each time. Values are presented as mean ± SEM, n = 3. a Values were obtained by dividing the calculated intensity of 5αR1 or HSO bands (27 cycles) by the calculated intensity of the 18S band (12 cycles). b Values were obtained by dividing the calculated intensity of 5αR2 bands (33 cycles) by the calculated intensity of the 18S band (12 cycles). c Values obtained from very faint bands, calculated as <0.1, were adjusted to 0.1.
