Table 1 Primer sequences and PCR conditions used to evaluate the expression of the transcripts indicated
Genes | Primers (5'-3') | Product size (bp) | Denaturing temperature and time (s) | Annealing temperature and time (s) | Extension temperature and time (s) | number of cycles |
|---|---|---|---|---|---|---|
IFNγR | F:ACgCAgAAggAAgATgATTgTgACg R:TCTATTggAgTCAgATggCTgCCC | 590 | 94°C (30) | 63°C (90) | 72°C (90) | 32 |
hNET | F:CTggTggTgAAggAgCgCAACggC R:ATgTgATgAATCCCgCTgCTCTCg | 241 | 94°C (60) | 63°C (60) | 72°C (60) | 32 |
β-actin | F:ATCATgTTTgAgACCTTCAACAC R:TCTgCgCAAgTTAggTTTTgTC | 822 | 94°C (60) | 63°C (60) | 72°C (60) | 32 |