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Table 1 Primer sequences and PCR conditions used to evaluate the expression of the transcripts indicated

From: A novel approach in the treatment of neuroendocrine gastrointestinal tumors: Additive antiproliferative effects of interferon-γ and meta-iodobenzylguanidine

Genes Primers (5'-3') Product size (bp) Denaturing temperature and time (s) Annealing temperature and time (s) Extension temperature and time (s) number of cycles
IFNγR F:ACgCAgAAggAAgATgATTgTgACg
R:TCTATTggAgTCAgATggCTgCCC
590 94°C (30) 63°C (90) 72°C (90) 32
hNET F:CTggTggTgAAggAgCgCAACggC
R:ATgTgATgAATCCCgCTgCTCTCg
241 94°C (60) 63°C (60) 72°C (60) 32
β-actin F:ATCATgTTTgAgACCTTCAACAC
R:TCTgCgCAAgTTAggTTTTgTC
822 94°C (60) 63°C (60) 72°C (60) 32
  1. All templates were initially denaturated for 5 min at 95°C and the amplification was followed by a final extension temperature of 72°C for 7 min.