Immunofluorescence of AIB1 and CBP in MDA-MB-436 (top row) and 436.1 (bracket) cells. Primary antibodies are as indicated. The cells were fixed at approximately 70% confluency and counterstained with DAPI (first panel). Red corresponds to AIB1 staining revealed with the Cy3-conjugated secondary goat anti-mouse antibody (second panel) and green corresponds to CBP staining with fluorescein isothiocyanate-conjugated goat anti-rabbit secondary antibody (third panel). The DAPI, AIB1, and CBP images from the same field of cells are merged in the last panel.