Figure 4

Hierarchical clustering of RNA expression profiles. Panel A clustering analysis of expression profiles of 8 MCF-7 sublines along with those of 19 breast cancer cell lines. Expression profiling was done using home made Nylon arrays comprising 721 cDNAs corresponding to identified genes localized on either chromosome 1q or 17q. Panel B clustering analysis of profiles of 7 MCF-7 sublines and the BT-474 breast cancer cell line. Nylon arrays comprised 1034 genes selected on the basis of their involvement in cancer. Clustering analysis wass done on raw quantification results, which were just subjected to a scaling step but not to ratio calculation. Parameters used in the analysis were Hierarchically Cluster Axes for Genesand Array: clusterand similarity metric correlation centered with average linkage clustering. The dendogram on top of the diagram represents cell lines ordered according to their degree of similarity. Complete datasets can be found at http://www.montp.inserm.fr/EMI0229/download