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Table 1 Immunohistochemical profile of both components of the tumor.

From: Post-radiation dedifferentiation of meningioma into osteosarcoma.

Immuno-marker Clone* Dilution Pretreatment / Target retrieval Meningioma component Osteosarcoma component
Vimentin Vim 3B4 1:200 Proteinase K enzyme 1:1 with TRIS buffer fro 4 min Immunoreactive Immunoreactive
CD34 QBEnd 10 1:25 10 mM citrate buffer, pH6 for 30 min with 20 min cool down Non-immunoreactive Non-immunoreactive
Pan-cytokeratin Polyclonal 1:50 Proteinase K enzyme 1:1 with TRIS buffer for 4 min Non-immunoreactive Non-immunoreactive
S-100 protein Polyclonal 1:300 Proteinase K enzyme 1:1 with TRIS buffer fro 4 min Immunoreactive Non-immunoreactive
Estrogen receptor ID5 1:75 10 mM citrate buffer, pH6 for 30 min with 20 min cool down Non-immunoreactive Non-immunoreactive
Progesteron receptor PgR636 1:50 10 mM citrate buffer, pH6 for 30 min with 20 min cool down. Focally immunoreactive Non-immunoreactive
EMA E29. (3). 1:50 No target retrieval Immunoreactive Non-immunoreactive
Ki-67 MIB-1 1:50 10 mM citrate buffer, pH6 for 30 min with 20 min cool down 12% nuclei immunoreactive 28% nuclei immunoreactive
  1. *All antibodies were from Dako, Carpinteria, CA.