Figure 3

Mitochondrial membrane potential changes and caspase activation. Panel A: Quotient of JC-1 red/green fluorescence as an indicator for the membrane potential ΔΨm in HCT116 cells and the bax-/- derivatives at different times after exposure to 100 μM resveratrol. Note that the absence of Bax delays but fails to completely inhibit membrane potential collapse. Standard deviations were derived from 4 experiments. Panel B: Detection of cleaved (active) caspases (casp) 9 and 3 in total protein extracts from parental and bax-deficient cells exposed to resveratrol. The anti-caspase antibodies were used at a dilution of 1:200. Staining with anti-β-actin is to show equal protein loading.