Figure 3

Effect of the ribozyme on the level of M6P/IGF2R mRNA in MCF-7 cells. A: a representative RT-PCR result showing the levels of M6P/IGF2R (lanes 3 and 4) and the corresponding amounts of β-actin mRNA (lane 1 and 2) in ribozyme-treated (lane 1, lane 3) and in control cells (lane 2, lane 4). B: bar chart showing percent change in M6P/IGF2R mRNA of ribozyme-treated cells relative to the mRNA fraction in control cells (normalized to β-actin). Total RNA was isolated from cultured MCF-7 cells infected with Ad.GFP/Rz-IGF2R or Ad.GFP for 72 hrs, and then subjected to RT-PCR using M6P/IGF2R specific primers and β-actin as an internal standard. The data are the mean ± SE of three separate experiments (n = 3, ^*, p < 0.05 versus control).