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Table 3 Profile of gene expression from 10 different experiments each using 5 single tumour cell/100 PBMNC aliquots from four different cell lines.

From: Optimisation of the RT-PCR detection of immunomagnetically enriched carcinoma cells

  MDA-MB-468 MDA-MB-453 MDA-MB-453 MDA-MB-453
  1 2 3 4 5 1 2 3 4 5 1 2 3 4 5 1 2 3 4 5
ELF3 + + + + + + + + + + + + + + + + + + + +
EphB4 + + + + + + + + + + + + + + + + + + + -
EpCam + + + + +       + + + + + + + + + +
EGFR + + + + + + - - + + - + - + - + + - - -
MGB1 - - - - -       - - - + - + - - + +
  T-47D T-47D T-47D T-47D
  1 2 3 4 5 1 2 3 4 5 1 2 3 4 5 1 2 3 4 5
ELF3 + + + + +       + + + + + + + - + -
EphB4 + + + + + - + + - + + + + + + + + - + -
EpCam + + + + + + + + + + + - + + + + + + + -
EGFR - - - - - - + - - - - - - - + + + + - -
MGB1 - - - - - - - - + + - - - - - + + - - -
  MCF-7 MCF-7           
  1 2 3 4 5 1 2 3 4 5           
ELF3 + + + + + + + + + +           
EphB4 - + + + + - + - + -           
EpCam - + + - + + + - + +           
EGFR - + - - - + - - - -           
MGB1 - - - - - - - - - -           
  1. Profile of gene expression from 10 different experiments each using 5 single tumour cell/100 PBMNC aliquots from four different cell lines demonstrating heterogeneity of gene expression and utility of several markers for accurate detection of the single tumour cell. A "+" indicates that marker was detected, a "-" indicates that marker was not detected, and a blank indicates that marker was not done.