Optimisation of the RT-PCR detection of immunomagnetically enriched carcinoma cells

BMC Cancer

Table 3 Profile of gene expression from 10 different experiments each using 5 single tumour cell/100 PBMNC aliquots from four different cell lines.

	MDA-MB-468					MDA-MB-453					MDA-MB-453					MDA-MB-453
	1	2	3	4	5	1	2	3	4	5	1	2	3	4	5	1	2	3	4	5
ELF3	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
EphB4	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	-
EpCam	+	+	+	+	+						+	+	+	+	+	+	+	+	+	+
EGFR	+	+	+	+	+	+	-	-	+	+	-	+	-	+	-	+	+	-	-	-
MGB1	-	-	-	-	-						-	-	-	+	-	+	-	-	+	+
	T-47D					T-47D					T-47D					T-47D
	1	2	3	4	5	1	2	3	4	5	1	2	3	4	5	1	2	3	4	5
ELF3	+	+	+	+	+						+	+	+	+	+	+	+	-	+	-
EphB4	+	+	+	+	+	-	+	+	-	+	+	+	+	+	+	+	+	-	+	-
EpCam	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+	-
EGFR	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	+	+	+	-	-
MGB1	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	+	+	-	-	-
	MCF-7					MCF-7
	1	2	3	4	5	1	2	3	4	5
ELF3	+	+	+	+	+	+	+	+	+	+
EphB4	-	+	+	+	+	-	+	-	+	-
EpCam	-	+	+	-	+	+	+	-	+	+
EGFR	-	+	-	-	-	+	-	-	-	-
MGB1	-	-	-	-	-	-	-	-	-	-

Profile of gene expression from 10 different experiments each using 5 single tumour cell/100 PBMNC aliquots from four different cell lines demonstrating heterogeneity of gene expression and utility of several markers for accurate detection of the single tumour cell. A "+" indicates that marker was detected, a "-" indicates that marker was not detected, and a blank indicates that marker was not done.

ISSN: 1471-2407