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Table 2 Comparison of the expression of the seven selected markers in either 100 PBMNCs only, or after the addition of a single tumor cell from one of four different cell lines.

From: Optimisation of the RT-PCR detection of immunomagnetically enriched carcinoma cells

 

ELF3

EpCam

EphB4

MUC1

CEA

EGFR

MGB1

Cycles

45

45

45

45

55

45

45

100 PBMNC

5/30

0/21

0/21

1/6

0/18

0/27

0/24

453

17/18

5/5

9/10

5/10

0/10

8/18

4/10

468

5/5

5/5

5/5

ND

ND

5/5

ND

MCF-7

10/10

8/10

6/10

5/5

9/10

1/5

1/10

T-47D

8/10

13/15

11/15

ND

ND

5/15

4/15

TOTAL

40/43

31/35

31/40

10/15

9/20

19/43

9/35

  1. Comparison of the expression of the seven selected markers in either 100 PBMNCs only, or after the addition of a single tumor cell from one of four different cell lines (MDA-MB-453, MDA-MB-468, MCF-7 and T47-D) after 45 or 55 rounds (CEA) of PCR amplification. For example EphB4 was detected in 9 out of 10 single MDA-MB-453 cells added to 100 PBMNC aliquots. ND- test not performed.