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Table 2 Comparison of the expression of the seven selected markers in either 100 PBMNCs only, or after the addition of a single tumor cell from one of four different cell lines.

From: Optimisation of the RT-PCR detection of immunomagnetically enriched carcinoma cells

  ELF3 EpCam EphB4 MUC1 CEA EGFR MGB1
Cycles 45 45 45 45 55 45 45
100 PBMNC 5/30 0/21 0/21 1/6 0/18 0/27 0/24
453 17/18 5/5 9/10 5/10 0/10 8/18 4/10
468 5/5 5/5 5/5 ND ND 5/5 ND
MCF-7 10/10 8/10 6/10 5/5 9/10 1/5 1/10
T-47D 8/10 13/15 11/15 ND ND 5/15 4/15
TOTAL 40/43 31/35 31/40 10/15 9/20 19/43 9/35
  1. Comparison of the expression of the seven selected markers in either 100 PBMNCs only, or after the addition of a single tumor cell from one of four different cell lines (MDA-MB-453, MDA-MB-468, MCF-7 and T47-D) after 45 or 55 rounds (CEA) of PCR amplification. For example EphB4 was detected in 9 out of 10 single MDA-MB-453 cells added to 100 PBMNC aliquots. ND- test not performed.