Table 6 Forward, reverse primers and probes used for ChIP-qPCR for amplification of the gene promoter region
Genes | qPCR primer sequences | qPCR MGB Probes (Taqman®) |
|---|---|---|
EZH2 | Forward AGTGCAATGGCGCGATCT | TCACCGCAACCTC |
Reverse GAGGCATGAGAATCGCTTGAA | ||
RAR beta 2 | Forward GCACGTAGGCTGTTGGTCTTT | CCAGCCCCGAATC |
Reversre GCTGGCTTGTCTGTCATAATTCA | ||
PGR | Forward GAGCCGCGTGTCACTAAATTG | CGTCGCAGCCGCA |
Reverse TCACAAGTCCGGCACTTGAG | ||
ER alpha | Forward CCCTGACATTGGCTTAAACATCA | TCCAGGCACAACTC |
Reverse TCTTTGGGATCGCTCCAAAT | ||
RGMA | Forward CTGCCAGGTCGGGAGTGT | AGAGGAGCAAGTTTG |
Reverse CACAGCCATAGGGCCTTCTC | ||
SRC3 | Forward AAAATTAAGGGCAGGGCTAGGA | TCCGGATCCCGAGGGAGCTCC |
Reverse GTGCGGCCGCTTTCG |