Figure 5

Effects of 3-deazaneplanocin A (DZNep) on KRT13 expression in OSCC cells. (A) SAS cells were treated with 10 μM DZNep for 24 h, and the levels of the PRC2 proteins (Ezh2 and Suz12) and H3K27me3 were examined by western blotting. Fold change in PRC2 proteins and H3K27me3 were normalized to β-actin and total histone H3, respectively, and calculated relative to band intensity of the control condition samples. The means ± SEM for each group (n = 3) are shown. Statistical analysis was performed using Student’s t-test. **p < 0.01. (B) KRT13 mRNA levels were analyzed by quantitative RT-PCR. Fold change in KRT13 mRNA was normalized to GAPDH and calculated relative to that of the cells under the control condition. The means ± SEM for each group (n = 3) are shown. Statistical analysis was performed using Student’s t-test. **p < 0.01. (C) H3K27me3 levels in the KRT13 promoter were analyzed by ChIP assay. Fold enrichment of histone H3K27me3 was normalized to that of total histone H3 and calculated relative to that of the cells under the control condition. The means ± SEM for each group (n = 3–5) are shown. Statistical analysis was performed using Student’s t-test. *p < 0.05.