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Table 6 Overview of median protein levels of uPA, uPAR and PAI-1 determined by different ELISA formats in malignant and non-malignant renal tissue specimens

From: Prognostic impact of urokinase-type plasminogen activator system components in clear cell renal cell carcinoma patients without distant metastasis

Parameter

Hofmann et al. [24],[25]

Swiercz et al. [23]a

Chautard et al. [22]b,c

Span et al. [32]

Present study

Number and type of Tu tissue samples

152 RCC (not specified): 105 for uPA, 49 for uPAR, 96 for PAI-1

52 RCC (not specified)

100 RCC: 85 ccRCC, 11 papillary RCC, 4 sarcomatoid

55 RCC (not specified)

112 ccRCC

Number and type of Tf tissue samples

matched normal tissues from RCC kidneys

n = 28: 3 RCC-free kidneys, 18 normal tissues from RCC-kidneys, 7 tissues adjacent to RCC

matched normal tissues from RCC kidneys

matched normal tissues from RCC kidneys

matched normal tissues from RCC kidneys

ELISA for uPA

American Diagnostica

American Diagnostica

BYK Sangtec Medical (Nichols Laboratory)

self-designed ELISA

American Diagnostica

ELISA for uPAR

American Diagnostica

American Diagnostica

n.d.

n.d.

American Diagnostica

ELISA for PAI-1

self-designed ELISA

American Diagnostica

American Diagnostica,

self-designed ELISA

American Diagnostica

Tissue processing

detergent tissue extracts

detergent tissue extracts

cytosolic tissue extracts

detergent tissue extracts

detergent tissue extracts

uPA

     

in Tu

0.29

n.p.

0.091 (0.043 in ccRCC)

0.50

0.41

in Tf

0.286

n.p.

0.078

0.71

0.61

Difference between Tu vs Tf

n.s. (Tu = Tf)

P = 0.01 (Tu > Tf)

n.s. (Tu > Tf)

n.s. (Tu < Tf)

P = 0.001 (Tu < Tf)

uPAR

     

in Tu

0.78

n.p.

n.d.

n.d.

0.51

in Tf

0.30

n.p.

n.d.

n.d.

0.52

Difference between Tu vs Tf

P = 0.018 (Tu > Tf)

P = 0.007 (Tu > Tf)

n.d.

n.d.

n.s. (Tu = Tf)

PAI-1

     

in Tu

11.08

n.p.

40.8 (38.7 in ccRCC)

7.26

10.3

in Tf

4.221

n.p.

5.8

0.92

3.13

Difference between Tu vs Tf

P = 0.02 (Tu > Tf)

P = 0.001 (Tu > Tf)

P < 0.001 (Tu > Tf)

P < 0.001 (Tu > Tf)

P = 0.001 (Tu > Tf)

  1. n.d.: not determined; n.p.: not provided; n.s.: not significant; Tf: non-malignant tissues; Tu: malignant tissues.
  2. aOnly P-values were given for differences between malignant and non-malignant tissue samples, which are presented in box plots.
  3. bResults are converted from pg/mg to ng/mg.
  4. cMean levels are shown since medians were not provided.
  5. Levels of uPA, uPAR and PAI-1 were normalised to total protein concentration and expressed as ng analyte per mg of total protein.
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BMC Cancer

ISSN: 1471-2407

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