Figure 4

HMGB1 expression in MDA-MB-231 cells treated with fibroblast CM. Real time PCR for HMGB1 expression in MDA-MB-231 cells treated with NTF-CMs and BCF-CMs for 48 h using paired fibroblasts isolated from the same patient.The levels of HMGB1 transcript (A) and protein levels (B) are shown after normalization against the internal control β-actin. Controls (Ctl) are cells cultured in fresh medium with no CM treatment. Bars represent the mean ± SD of triplicate experiments. ^$ = p-value of less than 0.05. * = p-value of less than 0.05 compared to the average HMGB1 of the two NTFs-CM treatment conditions whereas ^# = p-value of less than 0.05 compared to HMGB1 of the matched NTF-CM treatment.