Figure 5

Fibulin-5 exerts anti-metastatic effect by suppressing MMP-7 expression. A) The expression of MMP-7 was knocked down by a specific siRNA in HCC-LM3 cells. n = six independent experiments; *P < 0.05 by t test. B) Boyden chamber and Transwell assays indicated that MMP-7 knockdown inhibited migration and invasion of HCC-LM3 cells. n = three repeats with similar results, *P < 0.05 by t test. C) Fibulin-5-expressing HCC-LM3 cells that were transfected with empty vector (EV) or MMP-7 retroviruses were subjected to immunoblotting for MMP-7. n = six independent experiments; *P < 0.05 by t test. D) Restoring MMP-7 expression increased migration and invasion in Fibulin-5 expressing HCC-LM3 cells. n = three repeats with similar results, *P < 0.05 by t test.