Figure 3

Fibulin-5 reduces HCC cell migration and invasion. A) HCC-LM3 cells that were transfected with empty vector (EV) or Fibulin-5 retroviruses were subjected to western blot for Fibulin-5. n = six independent experiments; *P < 0.05 by t test. B) Cell migration and invasion as measured by Boyden chamber and Transwell assays were inhibited by Fibulin-5 overexpression in HCC-LM3 cells as compared with control cells. n = three repeats with similar results, *P < 0.05 by t test. HPF: high power field. C) MHCC97L cells that were transfected with scrambled siRNA or Fibulin-5 siRNA were subjected to western blot for Fibulin-5. n = six independent experiments; *P < 0.05 by t test. D) Fibulin-5 knockdown MHCC97L cells conferred a less number of migrated and invaded cells as compared with control cells. n = three repeats with similar results; *P < 0.05 by t test. HPF: high power field.