Representative images from immunohistochemical staining in different histological stages UNC5B was localized at cytoplasm and netrin-1 was mainly in cell nucleus and partly in cell cytoplasm of tumor tissues with granular brown staining. Almost all T1 tumor tissues showed strong UNC5B staining intensity. UNC5B expression in stage T4 was significantly less than in stage T1–T3 specimens (Figure 3A, B, C & D) (P = 0.021). While netrin-1 showed strong expression in stage T4 than other stages (T3, T2 & T1) and was associated significantly with pT stage (Figure 3E, F, G & H) (P = 0.013). Representative tissue sections with different immunointensity of UNC5B protein in A T1, B T2, C T3 & D T4. E Low level of netrin-1 expression in T1, H High level of netrin-1 expression in T4, F & G represent T2 &T3. Magnification × 400. *P < 0.05 compared with stage normal bladder tissues (paired t-test).