Figure 3
From: Fuse binding protein antagonizes the transcription activity of tumor suppressor protein p53
The transactivation activity of p53 is activated in FBP-kd Huh7 cells. (A) Activation of p53 transcription activity in FBP-kd Huh7 cells. Reporter vectors were transfected to control Huh 7 cells or FBP-kd cells as described in the Methods. Forty-eight hours later, cells were irradiated with 4 Gy of γ-irradiation. Luciferase activities were measured 6-hour post-irradiation treatment. p53 reporter activity (left panel), p21 reporter activity (right panel). (B) Transient expression of FBP1 in FBP-kd cells suppresses p53 transcription activities. FBP-kd cells were transfected with shRNA resistant FBP1 expression clone (pCIA-CMV-FBPSHR) along with reporter vectors; 48-h later cells were irradiated with 3 Gy of γ-irradiation. Luciferase activities were measured 6-hour post-irradiation treatment. p53 reporter activity (left panel), p21 reporter activity (right panel). (C) Relative p53 transcription activity in BCCIP-kd, p53-kd and FBP-kd cells. Huh7 cells knockdown for BCCIP; p53 and FBP1 were transfected with reporter vectors; 48-h later cells were irradiated with 3 Gy of γ-irradiation. Luciferase activities were measured 6-hour post-irradiation treatment. p53 reporter activity (left panel), p21 reporter activity (right panel).