Representative FISH images of the MCF7, BT474, MDA-MB361 and SKBR3 breast cancer cell lines using
/CEP17 dual-color probes. Metaphase spreads are shown and boxes indicate representative interphase nuclei for each case. FISH images for the BT474, MDA-MB361 and SKBR3 cells demonstrated HER2 and STARD3 gene amplification, while TOP2A gene amplification was observed in the SKBR3 cells only. Gene signals are red-labeled, CEP17 signals are green-labeled.