NA stimulates the proliferation of PC3 cells in an α
-AR and TRPV1 dependent manner. (A) The proliferation of PC3 cells was analysed by labelling with BrdU. The cells were treated with vehicle (control) or NA (100 μM) for 24 h, alone or in combination with WS433 (1 μM) and CPZ (1 μM). Cells were stained with an anti-BrdU FITC-conjugated Ab and analysed using a FACScan flow cytometer. Data shown are representative of one of three separate experiments. (B) siGLO-, siα1D-AR-, siTRPV1- and siα1D-AR/siTRPV1-transfected PC3 cells were treated for 24 h with NA (100 μM), and cell proliferation was assessed by BrdU labelling. The percentage increase of cell proliferation was evaluated with respect to vehicle-treated cells. The data shown are the mean ± SD of three separate experiments. Statistical analysis was performed by comparing siα1D-AR-, siTRPV1- with siGLO-transfected cells (*), and siα1D-AR/siTRPV1- with siα1D-AR- or siTRPV1-transfected cells (**), p ≤ 0.01.