Figure 7

Overexpression of h HSS1 impacts U87 and A172 tumor-induced HUVEC migration and invasion. A) Transwell migration and invasion assay for HUVEC co-cultured with U87 cells overexpressing hHSS1 or control vector. B) Transwell migration and invasion assay for HUVEC co-cultured with A172 cells overexpressing hHSS1 or control vector. Glioma cells were seeded in the bottom chamber containing media with 2% FBS. After 24 h, media was changed to serum-free media supplemented with 0.1% BSA. HUVEC were seeded in the upper chamber containing media with 0.1% BSA. A172 or U87 cells were seeded at 10:1 ratio of HUVEC. After 24 h, cells that migrated and invaded the matrix were fixed, stained with H&E and pictures (200x, magnification) of 21 fields of each replicate were taken for cells counting. Two independent experiments using duplicates were done for each assay. Data shown are mean ± SEM. ***P < 0.001, t-test. Black arrow shows net-like formation of invaded cells.