Top molecular networks of genes up- and down-regulated in U87 and A172 cells overexpressing
HSS1. Network of genes based on connectivity identified by IPA analysis. A) Top gene network of U87 cells depicting genes involved in cell cycle, cell death, DNA replication, recombination and repair. ANKRD1 was the most up-regulated gene. Many genes with a direct and indirect relationship with E2F gene were down-regulated by HSS1. B) Top gene network of A172-hHSS1 C#7 showing genes involved in cell cycle, cellular assembly and organization, DNA replication, recombination and repair. Several genes down-regulated by hHSS1 in A172 C#7 cells are target genes regulated by VEGF. C) Top gene network of A172-hHSS1 C#8 showing genes involved in tissue morphology and cellular development. Some of the hHSS1 modulated genes in A172 C#8 cells are responsible for ERK regulation. Different shapes of the nodes (genes/gene products) represent the functional classes of the gene products and the lines represent the biological relationships between the nodes. The length of an edge reflects the evidence in the literature supporting that node-to-node relationship. The intensity of the node color indicates the degree of up- (red) or down-regulation (green) of the respective gene. Gray represents a gene related to the others that did not meet the cutoff criteria. A solid line without arrow indicates protein-protein interaction. Arrows indicate the direction of action (either with or without binding) of one gene to another.