Differential miRNA expression profiles in malignant bile ducts and adjacent normal bile ducts. A. Unsupervised hierarchical clustering was performed using log2 relative frequencies of 29 differentially expressed miRNAs. Red represents overexpressed miRNAs; green represents underexpressed miRNAs. B. Endogenous levels of miR-144 in HCCC-9810, CCLP1, HuCC-T1, RBE, and BEC cells detected by q-PCR analysis. C. Relative expression of miR-144 in CCA tissues was significantly lower than that in corresponding normal bile duct tissues. Tumor indicates CCA tissue samples, and non-tumor indicates adjacent normal bile ducts. U6 was used as a loading control. *P < 0.05, **P < 0.01.