Figure 4

Distribution of the eEF1H subunits in A549 cells. (A) Subcellular fractionation. CE - cytoplasmic extract, NM – nuclear membrane fraction, NE – nuclear extract, C — control (the pellet after taking out the cytoplasmic fraction and before isolation of nuclei). (B) Immunofluorescent analysis of eEF1Bγ localization. (a) Localization of eEF1Bγ in A549 cells, (b) nuclear staining with Hoechst 33342, (c) merge. Scale bar 20 μm. (C) Co-immunoprecipitation of eEF1A and eEF1Bγ. CE – cytoplasmic extract, NE – nuclear extract. IP – fraction of immunoprecipitated proteins, B – fraction of non-bound proteins.