Figure 7
From: Enhanced detection of metastatic prostate cancer cells in human plasma with lipid bodies staining
Effect of fixation on lipophilic dye retention in LNCaP and PBMC cells. (A) Both LNCaP and PBMC cells were labeled with Bodipy for 5 min, after labeling half of the cells were fixed and permeabilized using intrasure kit. (B) Permeabilized cells were labeled with pan-cytokeratin antibody tagged with Alexa647 for 30 min at room temperature. (B) Blue lines represent LNCaP or PBMC cells without staining with Bodipy FL or immunolabeled with pan-cytokeratin antibody tagged with Alexa 647. Red lines represent LNCaP or PBMC cells stained with Bodipy FL or immunolabeled with pan-cytokeratin antibody tagged with Alexa 647. (C) Two-color flow cytometry data for dual-stained LNCaP and PBMC cells. Data presented are of one set of flow cytometry experiments. (D) The average values of four experiments are shown in a graphical format. Asterisk indicates P-value < 0.05 determined with paired Student’s t-test against untreated control.