Figure 3

Inhibition of miR-1269 suppresses the proliferation and tumorigenicity of HCC cells. A. Real-time PCR analysis miR-1269 in BEL-7404 and Huh7 cells transfected with a miR-1269 inhibitor. Transcript levels were normalized to U6 expression. B. The effects of miR-1269 inhibition on cell viability of the indicated cell lines analyzed by MTT assay. C. Representative micrographs (left panel) and quantification (right panel) of crystal violet stained colonies formed by the indicated cell lines. D. Effects of miR-1269 inhibition on the tumorigenicity of the indicated cell lines determined by anchorage-independent growth ability assay. E. The effect of miR-1269 on cell cycle progression of the indicated cell lines analyzed by flow cytometry. Each bar represents the mean ± SD of three independent experiments. * P < 0.05.