Figure 4From: Epigenetic inactivation of ST6GAL1 in human bladder cancer ST6GAL1 promoter hypermethylation in primary human bladder cancer is associated with loss of ST6GAL1 mRNA expression. (A) Representative MSP results of the ST6GAL1 promoter methylation status in four papillary non-invasive pTa low grade tumors (PAP) as well as four invasive high grade bladder cancer (INV) samples in comparison to four normal tissue specimens (NU). Bands labeled with U and M show unmethylated and methylated DNA, respectively. Percent values reflect methylation frequency (M frequency). Bisulphite-converted unmethylated, genomic (U-co) and polymethylated, genomic (M-co) DNA were used as positive controls. DNA control: genomic, non- bisulphite-converted DNA, NTC: non-template control. (B) Box plot illustrating ST6GAL1 mRNA downregulation according to hypermethylated ST6GAL1 promoter status in bladder cancer (UC). (U): unmethylated tumors. (M): Methylated tumors. Horizontal lines: grouped medians. Boxes: 25–75% quartiles. Vertical lines: range, peak and minimum; *p < 0.05.Back to article page