Figure 2

ST6GAL1 protein expression in human bladder cancer. (A) + (B) matched samples of patient #1 with (A) normal urothelium with accentuated ST6GAL1 protein expression in superficial (“umbrella”) cells and (B) complete loss of ST6GAL1 protein in the poorly differentiated invasive bladder cancer. (C) + (D) Matched samples of patient #2 with (C) normal urothelium with accentuated ST6GAL1 protein expression in superficial (“umbrella”) cells and (D) moderate ST6GAL1 protein expression in the invasive bladder cancer (staining intensity 2). (E) Positive control: prostate sample with strong ST6GAL1 staining (staining intensity 3). (F) Negative control: prostate sample with no staining (staining intensity 0). (G) Histogram of ST6GAL1 protein expression (immunoreactive score, IRS) distribution among all tumor samples.