Figure 4

Effects of CaM antagonists on apoptosis in multiple myeloma cells. Cells were treated with CaM antagonists (60 μM) for 24 h. The cells were subsequently harvested and incubated with FITC-labeled annexin V and propidium iodide and analyzed by flow cytometry. In the representative flow plots, the lower left quadrant contains normal cells; the lower right quadrant contains early apoptotic cells; and the upper right quadrant contains late apoptotic and necrotic cells.