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Figure 2 | BMC Cancer

Figure 2

From: MTDH mediates trastuzumab resistance in HER2 positive breast cancer by decreasing PTEN expression through an NFκB-dependent pathway

Figure 2

Elevated MTDH and reduced PTEN expressions in trastuzumab-resistant HER2 positive breast cancer cells. A. Relative proliferation of SK-BR-3 and SK-BR-3/R cells under trastuzumab exposure (5 μg/ml) at different time points by MTT assay (*P < 0.05); B. Dose-dependent trastuzumab cytotoxicity in SK-BR-3 cells by MTT assay (*P < 0.05). The proliferation curve of SK-BR-3/R cells was relatively flat, indicating trastuzumab resistance; C. Real-timeRT-PCR revealed mRNA expression of MTDH is significantly higher in SK-BR-3/R cells, while PTEN expression on the opposite (*P < 0.05); D. Higher levels of MTDH and p-Akt expressions as well as reduced PTEN expression were detected in SK-BR-3/R cells by western blot analysis; E. Significant differences in MTDH, PTEN and p-AKt expressions between SK-BR-3 and SK-BR-3/R cells (*P < 0.05); F. The immunofluorescence staining of SK-BR-3 and SK-BR-3/R cells revealed differential MTDH, PTEN and p-Akt expressions. All in vitro experiments were repeated for three times.

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BMC Cancer

ISSN: 1471-2407

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