Figure 3

STATIP1 mRNA depletion by siRNA induces the over-expression of STAT3 and its target genes. (A) STATIP1 mRNA levels at 24 h, 48 h and 72 h after STATIP1 silencing, as determined by RT-qPCR. (B) Western blot analyses of the STATIP1 protein level 72 h after STATIP1 silencing. (C) RT-qPCR analyses of the STAT3 mRNA levels at 24 h, 48 h and 72 h after STATIP1 silencing. (D) RT-qPCR analyses of the CCND1 and BCL-XL mRNA levels at 72 h after STATIP1 silencing. All comparisons were made to untreated cells – ctrl and scrambled-treated cells. Ctrl: control. The data represent the means ± SD of at least three independent experiments (*p <0.05 and **p <0.01).