Progressive loss of Notch inhibition. Pancreata were harvested at the indicated time points from 15-week (A) and 26-week old (B) wild type (WT), p48Cre;LSL-Kras
G12D (KC), and p48Cre;LSL-Kras
DNMAML-GFP/+ (KC;DNMAML) mice. Immunofluorescence images represent Notch activity (Hes1; red; 80×) and DNMAML expression (GFP; green; 60×). To identify the nucleus, all sections were stained with DAPI. (C) Quantification of DNMAML expression (GFP) within the nucleus or within the cytoplasm at 15 weeks and 26 weeks. Data represents mean ± SEM. ***, P < 0.001. (D) RT-qPCR analysis of Hedgehog signaling component, Patched 1(Ptch1) and Wnt ligand, Wnt3a (Wnt3a) in KC and KC;DNMAML mice at the indicated time points. Each point represents 1 mouse. Data represents mean ± SEM. Gapdh and Cyclophilin were used as reference genes.