Figure 2
From: Selective inhibition of RET mediated cell proliferation in vitro by the kinase inhibitor SPP86
SPP86- mediated RET inhibition does not abolish its phosphorylation TPC1 cells. (A) TPC1 cells were cultured overnight in media containing 0.1% FBS and exposed to 2.5 μM PF573228 and/or 1 μM SPP86 for 90 min in similar media. Total lysates were resolved by SDS-PAGE and probed with antibodies directed against phosphorylated (Tyr1062) and total RET. Actin was used to monitor gel loading. (B- D) Total lysates were resolved by SDS-PAGE and probed with antibodies directed against the indicated proteins. Tubulin was used to monitor gel loading. (E) TPCI cells were grown in media containing 0.1% FBS and the left untreated or cultured in the presence of 2.5 μM PF573228 and/or 1 μM SPP86 for 72 h. Viability was expressed as a percentage of the untreated control population. The data in each panel represent the mean of 3 experiments ± S. E.; *p <0.01 treated vs. untreated for each series.