Figure 6

The FAST2 domain mediates the apoptotic effect of FASTKD2. HeLa cells were transfected with 50 ng of vectors that express either FASTKD2, FASTKD2(1–455) lacking the FASTKD2 FAST1_FAST2 and RAP domains, FASTKD2(456–619) which contains the FAST1_FAST2 domain or FASTKD2(538–619) which contains just the FAST2 domain. Fifteen h later cells were fixed and permeablized for TUNEL assay (red) and fluorescence studies (green). Nuclei were stained with DAPI (blue). FASTKD2 and FASTKD2(1–455) were detected by immunofluorescence using FLAG-M2 antibody while expression of the other FASTKD2 domains were detected as GFP chimeras.