Figure 5

FOXP1 re-expression inhibits growth of neuroblastoma cells. FoxP1-induced changes in (a) cell viability (Trypan Blue dye exclusion analysis 6 days after induction of transgene expression), (b) cell proliferation (Alamar Blue assay 6 days after induction of transgene expression), (c) the proportion of cells showing apoptotic nuclei (72 h after induction of transgene expression), (d) the proportion of Annexin-V-positive cells (fluorescence-activated cell sorting (FACS)) and (e) cell cycle distribution (FACS). Each value represents the mean of triplicate experiments; error bars indicate S.D.