Figure 5

miR-26a directly targets AMACR. (A) The mature miR-26a sequence aligned with the predicted binding site within the 3′UTR of AMACR is depicted. (B) Luciferase activity was measured in DU-145 cells co-transfected with 100 nM miR-26a mimic and 1.5 μg of the wildtype (pmir-GLO-A26a) or mutated (pmir-GLO-Amut26a) luciferase reporter vector for AMACR. Normalized values are shown relative to the control treatment with 100 nM miR-CON and the wildtype luciferase reporter vector (100%). Values represent averages of six independent experiments with their mean deviation. A one-tailed paired t-test was used to compare differences between the respective treatments and the control treated cells (pmir-GLO-A26a + miR-CON): *p < 0.05.