Figure 1

Baseline abundance of insulin signalling proteins in human primary pancreatic ductal cells, human HPDE cells and human PANC1 cells. Relative expression of Insulin receptor β (InsRβ), IGF1 receptor (IGF1R), phosphorylated AKT at S473 (p-AKT), total AKT (AKT), phosphorylated ERK (p-ERK), and total ERK (ERK) were examined under basal conditions. From the left to right, there are three biological independent primary ductal cells samples, four HPDE cells samples and four PANC1 cells samples from different passages. Note the uneven loading of primary ductal samples as indicated by the actin loading control, which prevents quantitative comparisons. Other than lanes 1 and 2, every effort was made to load an equal amount of protein into each lane.